prof. UAM dr hab. Jacek Gapiński
profesor uczelni, kierownik zakładu
e-mail: jacek.gapiński@amu.edu.pl
tel.: +48 61 829 5265
pokój: 205/C
Zainteresowania naukowe
- Struktura i dynamika miękkiej materii
- Optyczne metody badania dyfuzji w ośrodkach prostych i złożonych
- Egzotyczna mikroskopia optyczna (LSM, TIRF, FLIM, up-conversion)
Wykształcenie
- III Liceum Ogólnokształcące w Poznaniu, 1985
- magister fizyki, Wydział Matematyki i Fizyki UAM w Poznaniu, 1990
- doktor fizyki, Wydział Matematyki i Fizyki UAM w Poznaniu, 1994
- habilitacja z fizyki, Wydział Fizyki UAM w Poznaniu, 2010
Inne informacje
Zatrudnienie: 1988: pomoc techniczna w ZBM; 1990: asystent w ZBM; 1994: adiunkt w ZBM; 2012: prof. UAM w ZBM
Staże zagraniczne:
- ok. 2 lat w postaci 1-4 miesięcznych wyjazdów do Max-Planck-Institut fuer Polymerforschung w Moguncji, Niemcy (grupa prof. E. W. Fischera)
- ok. roku w postaci 1-3 miesięcznych wyjazdów do Forschungszentrum Juelich, Niemcy (grupa prof. J. Dhonta)
- ok. roku w postaci 1-3 miesięcznych wyjazdów do Research Center of Crete, Grecja (prof. G. Fytas, D. Vlassopoulos)
- 3 wyjazdy 30-dniowe do Stanford University (Wydział Chemii, prof. Robert Pecora)
Wypromowani doktorzy:
- Tomasz Śliwa (2015) „Badania nad strukturą i właściwościami polimerów zbudowanych z poli(N-izopropyloakryloamidu) i ich potencjalne zastosowanie w medycynie”
Nagrody i wyróżnienia:
- Liczne Nagrody Rektora UAM
Przynależność do Towarzystw Naukowych:
- Polskie Towarzystwo Fizyczne, Polskie Towarzystwo Biofizyczne
2021
Richter, Łukasz; Księżarczyk, Karolina; Paszkowska, Karolina; Janczuk-Richter, Marta; Niedziółka-Jönsson, Joanna; Gapiński, Jacek; Łoś, Marcin; Hołyst, Robert; Paczesny, Jan
Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research Journal Article
In: Scientific Reports, vol. 11, no. 1, pp. 7387, 2021, ISSN: 20452322.
Abstract | Links | BibTeX | Tagi: Bacteriophages, Biomedical engineering, Design, Nanobiotechnology, Polymers, Surface chemistry, synthesis and processing
@article{Richter2021,
title = {Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research},
author = {Łukasz Richter and Karolina Księżarczyk and Karolina Paszkowska and Marta Janczuk-Richter and Joanna Niedziółka-Jönsson and Jacek Gapiński and Marcin Łoś and Robert Hołyst and Jan Paczesny},
url = {https://www.nature.com/articles/s41598-021-86571-x},
doi = {10.1038/s41598-021-86571-x},
issn = {20452322},
year = {2021},
date = {2021-12-01},
journal = {Scientific Reports},
volume = {11},
number = {1},
pages = {7387},
publisher = {Nature Research},
abstract = {Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container's wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.},
keywords = {Bacteriophages, Biomedical engineering, Design, Nanobiotechnology, Polymers, Surface chemistry, synthesis and processing},
pubstate = {published},
tppubtype = {article}
}
Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container's wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions' adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.