prof. UAM dr hab. Jacek Gapiński
Zainteresowania naukowe
- Struktura i dynamika miękkiej materii
- Optyczne metody badania dyfuzji w ośrodkach prostych i złożonych
- Egzotyczna mikroskopia optyczna (LSM, TIRF, FLIM, up-conversion)
Wykształcenie
- III Liceum Ogólnokształcące w Poznaniu, 1985
- magister fizyki, Wydział Matematyki i Fizyki UAM w Poznaniu, 1990
- doktor fizyki, Wydział Matematyki i Fizyki UAM w Poznaniu, 1994
- habilitacja z fizyki, Wydział Fizyki UAM w Poznaniu, 2010
Inne informacje
Zatrudnienie: 1988: pomoc techniczna w ZBM; 1990: asystent w ZBM; 1994: adiunkt w ZBM; 2012: prof. UAM w ZBM
Staże zagraniczne:
- ok. 2 lat w postaci 1-4 miesięcznych wyjazdów do Max-Planck-Institut fuer Polymerforschung w Moguncji, Niemcy (grupa prof. E. W. Fischera)
- ok. roku w postaci 1-3 miesięcznych wyjazdów do Forschungszentrum Juelich, Niemcy (grupa prof. J. Dhonta)
- ok. roku w postaci 1-3 miesięcznych wyjazdów do Research Center of Crete, Grecja (prof. G. Fytas, D. Vlassopoulos)
- 3 wyjazdy 30-dniowe do Stanford University (Wydział Chemii, prof. Robert Pecora)
Wypromowani doktorzy:
- Tomasz Śliwa (2015) „Badania nad strukturą i właściwościami polimerów zbudowanych z poli(N-izopropyloakryloamidu) i ich potencjalne zastosowanie w medycynie”
Nagrody i wyróżnienia:
- Liczne Nagrody Rektora UAM
Przynależność do Towarzystw Naukowych:
- Polskie Towarzystwo Fizyczne, Polskie Towarzystwo Biofizyczne
2021
Litowczenko, Jagoda; Gapiński, Jacek; Markiewicz, Roksana; Woźniak, Anna; Wychowaniec, Jacek K; Peplińska, Barbara; Jurga, Stefan; Patkowski, Adam
Synthesis, characterization and in vitro cytotoxicity studies of poly-N-isopropyl acrylamide gel nanoparticles and films Journal Article
In: Materials Science and Engineering: C, vol. 118, pp. 111507, 2021, ISSN: 0928-4931.
Abstract | Links | BibTeX | Tagi: Cell cytotoxicity, Dynamic light scattering, Films, Hydrogels, Poly-N-isopropyl acrylamide (pNIPAM), Swelling and deswelling properties
@article{LITOWCZENKO2021111507,
title = {Synthesis, characterization and in vitro cytotoxicity studies of poly-N-isopropyl acrylamide gel nanoparticles and films},
author = {Jagoda Litowczenko and Jacek Gapiński and Roksana Markiewicz and Anna Woźniak and Jacek K Wychowaniec and Barbara Peplińska and Stefan Jurga and Adam Patkowski},
url = {http://www.sciencedirect.com/science/article/pii/S0928493120334251},
doi = {https://doi.org/10.1016/j.msec.2020.111507},
issn = {0928-4931},
year = {2021},
date = {2021-01-01},
journal = {Materials Science and Engineering: C},
volume = {118},
pages = {111507},
abstract = {In this work, we show synthesis that leads to thermoreponsive poly-N-isopropyl acrylamide (pNIPAM) nanogels with sizes below 100 nm, irrespectively of the surfactant to crosslinker ratio. We also show that in many environments the temperature induced pNIPAM collapse at Lower Critical Solution Temperature (LCST) of 32.5 °C is accompanied by gel nanoparticles' aggregation. Thus, the proper information on the nanoparticle (NP) structure and deswelling can be obtained only if the routinely measured hydrodynamic radius is supplemented by information on the molecular weight, which can be obtained from the intensity of scattered light. We measured the dynamics and reversibility of the deswelling and subsequent aggregation processes. Furthermore, we show that the highly concentrated pNIPAM gel NPs reversibly form bulk hydrogel networks of varied interconnected porous structure. We show, that in case of drying pNIPAM gel NPs above the LCST, it is possible to obtain films with 20-fold increase in storage modulus (G′) compared to hydrogel networks measured at room temperature. They exhibit temperature hysteresis behavior around LCST of 32.5 °C similar to pNIPAM films. Finally, we show that these hydrogel films, lead to extended proliferation of cells across three different types: fibroblast, endothelial and cancer cells. Additionally, none of the films exhibited any cytotoxic effects. Our study brings new insights into physicochemical characterization of pNIPAM gel NPs and networks behavior in realistic conditions of in vitro measurements, especially by means of dynamic light scattering as well as final unique properties of both gel NPs and formed porous films for possible tissue engineering applications.},
keywords = {Cell cytotoxicity, Dynamic light scattering, Films, Hydrogels, Poly-N-isopropyl acrylamide (pNIPAM), Swelling and deswelling properties},
pubstate = {published},
tppubtype = {article}
}
2019
Jarzębski, Maciej; Peplińska, Barbara; Florczak, Patryk; Gapiński, Jacek; Flak, Dorota; Mała, Patrycja; Ramanavicius, Arunas; Baryła-Pankiewicz, Elżbieta; Cisowska, Joanna Kobus-; Szwajca, Anna
Fluorescein ether-ester dyes for labeling of fluorinated methacrylate nanoparticles Journal Article
In: Journal of Photochemistry and Photobiology A: Chemistry, vol. 382, pp. 111956, 2019, ISSN: 1010-6030.
Abstract | Links | BibTeX | Tagi: Dynamic light scattering, Fluorescence correlation spectroscopy, Fluorescent fluorescein-derivatives, Fluorescent nanoparticles, Fluorinated nanoparticless, Nanoparticle tracking analysis
@article{JARZEBSKI2019111956,
title = {Fluorescein ether-ester dyes for labeling of fluorinated methacrylate nanoparticles},
author = {Maciej Jarzębski and Barbara Peplińska and Patryk Florczak and Jacek Gapiński and Dorota Flak and Patrycja Mała and Arunas Ramanavicius and Elżbieta Baryła-Pankiewicz and Joanna Kobus- Cisowska and Anna Szwajca},
url = {http://www.sciencedirect.com/science/article/pii/S1010603019304058},
doi = {https://doi.org/10.1016/j.jphotochem.2019.111956},
issn = {1010-6030},
year = {2019},
date = {2019-01-01},
journal = {Journal of Photochemistry and Photobiology A: Chemistry},
volume = {382},
pages = {111956},
abstract = {Fluorescent core-shell particles are used as versatile fluorophores in confocal microscopy based image analysis and as a colloidal model system to study short and long-range interactions. Bright and stable microspherical probes are proposed as promising materials, especially in bioimaging applications. The release of dyes from fluorescent polymer microspheres is undesirable. Covalent linking of dyes within polymeric spheres during the polymerization process can solve the problem of dye leaching. This requires, e.g. the introduction of reactive groups into the dyes. Its more lipophilic ester-ether derivatives considerably reduced fluorescence. The fluorescent quantum yield of prepared nanoparticles was determined to be below 10%. As-prepared nanoparticles exhibited a low refractive index (1.293–1.349), hence their use is recommended. Scanning electron microscope (SEM) images and the fluorescence correlation spectroscopy (FCS) measurements confirmed high polydispersity of synthesized particles (40–230 nm), and are in agreement with the dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) results (hydrodynamic diameters 203 ± 9, 548 ± 50, 146 ± 2 nm). The zeta potential of fluorescent 1H,1H-heptafluoro-n-butyl methacrylate (HFBMA) shell nanoparticles (NPs) with propargyl ether-esters, 2-methyl allyl ether-esters and allyl ether-esters of fluorescein was -44.5,-14, -44.7 mV, respectively. The values are different despite the slight difference in the structure of ester-ether derivatives.},
keywords = {Dynamic light scattering, Fluorescence correlation spectroscopy, Fluorescent fluorescein-derivatives, Fluorescent nanoparticles, Fluorinated nanoparticless, Nanoparticle tracking analysis},
pubstate = {published},
tppubtype = {article}
}